Transformed olive calli produced from cv. Dolce Agogia leaf petioles were obtained using Agrobacterium tumefaciens LBA4404 carrying the plasmid pBIN19 containing rol A, B, C (ORFs 10, 11, 12) from A. rhizogenes T-DNA. The presence of the DNA insert in the callus genome was shown by its resistence to kanamycin, by its positivity to the neomicyn phosphotranferase II (NPT II) enzyme activity assay and by Southern hybridization. Transgenic callus was able to regenerate roots in low auxinic medium. The resilts, a first for the species, indicate the possibility of transforming olive and of using rol genes to improve the rooting ability of some olive cultivars.
Agrobacterium-mediated DNA trasfer in olive callus (Olea europaea L.).
MICHELI, Maurizio;
1999
Abstract
Transformed olive calli produced from cv. Dolce Agogia leaf petioles were obtained using Agrobacterium tumefaciens LBA4404 carrying the plasmid pBIN19 containing rol A, B, C (ORFs 10, 11, 12) from A. rhizogenes T-DNA. The presence of the DNA insert in the callus genome was shown by its resistence to kanamycin, by its positivity to the neomicyn phosphotranferase II (NPT II) enzyme activity assay and by Southern hybridization. Transgenic callus was able to regenerate roots in low auxinic medium. The resilts, a first for the species, indicate the possibility of transforming olive and of using rol genes to improve the rooting ability of some olive cultivars.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
