Mature β-hexosaminidase A has been found associated to the external leaflet of plasma membrane of cultured fibroblasts. The plasma membrane association of β-hexosaminidase A has been directly determined by cell surface biotinylation followed by affinity chromatography purification of the biotinylated proteins, and by immunocytochemistry. The immunological and biochemical characterization of biotinylated β-hexosaminidase A revealed that the plasma membrane associated enzyme is fully processed, suggesting its lysosomal origin.
Identification of plasma membrane associated mature B-hexosaminidase A, active towards GM2 ganglioside, in human fibroblasts
MENCARELLI, Simona;CAVALIERI, Cristina;MAGINI, Alessandro;TANCINI, Brunella;ORLACCHIO, Aldo;EMILIANI, Carla;
2005
Abstract
Mature β-hexosaminidase A has been found associated to the external leaflet of plasma membrane of cultured fibroblasts. The plasma membrane association of β-hexosaminidase A has been directly determined by cell surface biotinylation followed by affinity chromatography purification of the biotinylated proteins, and by immunocytochemistry. The immunological and biochemical characterization of biotinylated β-hexosaminidase A revealed that the plasma membrane associated enzyme is fully processed, suggesting its lysosomal origin.File in questo prodotto:
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