β -mannosidase is an exoglycosidase involved in the degradation of N-linked oligosacharides moieties of glycoproteins. Lack of β -mannosidase activity leads to the lysosomal disorder β -mannosidosis (MIM 248510). We have isolated and sequenced the gene encoding the mouse β -mannosidase. Comparison of the deduced amino acid sequence of mouse, human, bovine, and goat β -mannosidase showed 64% identity, reflecting a high degree of evolutionary conservation. Analysis of a multiple tissue northern blotting revealed a major transcript of about 3.7 kb in all tissues examined. The northern analysis also demonstrates that there is differential tissue mRNA expression. The mouse β -mannosidase gene (Bmn) was mapped to the distal end of Chromosome (Chr) 3, in a region that is homologous with a segment of human Chr 4 containing the orthologous human gene.
Mouse beta-mannosidase: cdna cloning, expression, and chromosomal localization
BECCARI, Tommaso;BIBI, Lucia;STINCHI, Sofia;ORLACCHIO, Aldo
2001
Abstract
β -mannosidase is an exoglycosidase involved in the degradation of N-linked oligosacharides moieties of glycoproteins. Lack of β -mannosidase activity leads to the lysosomal disorder β -mannosidosis (MIM 248510). We have isolated and sequenced the gene encoding the mouse β -mannosidase. Comparison of the deduced amino acid sequence of mouse, human, bovine, and goat β -mannosidase showed 64% identity, reflecting a high degree of evolutionary conservation. Analysis of a multiple tissue northern blotting revealed a major transcript of about 3.7 kb in all tissues examined. The northern analysis also demonstrates that there is differential tissue mRNA expression. The mouse β -mannosidase gene (Bmn) was mapped to the distal end of Chromosome (Chr) 3, in a region that is homologous with a segment of human Chr 4 containing the orthologous human gene.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
