The interaction between stem cells and biomaterials with nanoscale topography represents a main route in the roadmap for tissue engineering-based strategies. In this study, we explored the interface between human bone marrow-derived mesenchymal stem cells (hBM-MSCs) and hydrogenated amorphous carbon (a-C:H) film designed with uniform, groove, or grid nanopatterns. In either case, hBM-MSCs preserved growth rate and multi- differentiation properties, suggesting that the films were biocompatible and suitable for stem cell culture. hBM-MSCs responded to different nanopattern designs with specific changes of microtubule organization. In particular, the grid pattern induced a square-localized distribution of alpha-tubulin/actin fibers, whereas the groove pattern exerted a more dynamic effect, associated with microtubule alignment and elongation.

Hydrogenated Amorphous Carbon Nanopatterned Film Designs Drive Human Bone Marrow Mesenchymal Stem Cell Cytoskeleton Architecture

MARTINO, Sabata;ARMENTANO, ILARIA;TIRIBUZI, ROBERTO;PENNACCHI, Manuela;DOTTORI, MARIASERENA;MATTIOLI, SAMANTHA;CARAFFA, Auro;CERULLI, Giuliano Giorgio;KENNY, Jose Maria;ORLACCHIO, Aldo
2009

Abstract

The interaction between stem cells and biomaterials with nanoscale topography represents a main route in the roadmap for tissue engineering-based strategies. In this study, we explored the interface between human bone marrow-derived mesenchymal stem cells (hBM-MSCs) and hydrogenated amorphous carbon (a-C:H) film designed with uniform, groove, or grid nanopatterns. In either case, hBM-MSCs preserved growth rate and multi- differentiation properties, suggesting that the films were biocompatible and suitable for stem cell culture. hBM-MSCs responded to different nanopattern designs with specific changes of microtubule organization. In particular, the grid pattern induced a square-localized distribution of alpha-tubulin/actin fibers, whereas the groove pattern exerted a more dynamic effect, associated with microtubule alignment and elongation.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11391/171379
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