The potential allelochemical, antimicrobial and antioxidant activities of MeOH extracts of Tuber borchii, T. aestivum, andT. brumale f. moschatum were investigated. The allelopathic effect of the truffle extracts was evaluated\using a Petri dish assay. Twenty seeds of each plant species (Hieracium pilosella, Lotus corniculatus, Melica ciliata, Silene vulgaris) were sown on filter paper in a Petri dish with 7.5 ml of the test solution of the MeOH extracts. The antibacterial activity of the truffle MeOH extract against Streptomyces species was determined by broth microdilution method. The MeOH extracts of truffle had a dose-dependent inibitory effect on seedling height and root length and showed significant antibacterial activity against Streptomyces species. The antioxidant activity of the methanolic extracts was tested with the DPPH test. The results showed that between 38.7 and 19.2 mg were necessary to reduce 50% of the DPPH coloration. There was no linear correlation between antioxidant activities and the polyphenol content.
Biological activities of methanolic extract from Tuber aestivum, T. borchii, and T. brumale f. moschatum .
ANGELINI, Paola;DONNINI, Domizia;PAGIOTTI, Rita;GRANETTI, Bruno;VENANZONI, Roberto
2010
Abstract
The potential allelochemical, antimicrobial and antioxidant activities of MeOH extracts of Tuber borchii, T. aestivum, andT. brumale f. moschatum were investigated. The allelopathic effect of the truffle extracts was evaluated\using a Petri dish assay. Twenty seeds of each plant species (Hieracium pilosella, Lotus corniculatus, Melica ciliata, Silene vulgaris) were sown on filter paper in a Petri dish with 7.5 ml of the test solution of the MeOH extracts. The antibacterial activity of the truffle MeOH extract against Streptomyces species was determined by broth microdilution method. The MeOH extracts of truffle had a dose-dependent inibitory effect on seedling height and root length and showed significant antibacterial activity against Streptomyces species. The antioxidant activity of the methanolic extracts was tested with the DPPH test. The results showed that between 38.7 and 19.2 mg were necessary to reduce 50% of the DPPH coloration. There was no linear correlation between antioxidant activities and the polyphenol content.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
