The encapsulation technology represents a new tool to integrate micropropagation into the nursery activity. It allows to combine the advantages of zygotic or gamic seeds with those of micropropagation. One of the products of this technology is represented by synthetic seed or artificial seed, described as "artificially encapsulated somatic embryos, shoots or other tissues which can be used for sowing under in vitro or ex vitro conditions" (Aitken-Christie et al. 1995). This technique presents some problems connected to the low regrowth and conversion (formation of entire plantlet) rates of the encapsulated propagules. Synthetic seeds will be a powerful propagation tool in the nurseryman hands, if the levels of the synthetic seeds conversion will be increased also in the nurseries, without the asepsis of in vitro laboratories and with the presence of many parasitic microorganisms, like bacteria and fungi, responsible for contamination and/or for trophic competition. This research has been carried out in order to introduce the biotization to the synthetic seed technology of Carrizo citrange [C. sinensis (L.) Osb. x Poncirus trifoliata (L.) Raf.], one of the most widespread citrus rootstocks, because of its resistance to the Citrus Tristeza Virus (CTV). With this goal, preliminary experiments to set up protocols for biotization, through the introduction of Plant Growth Promoting Bacteria (PGPB) and of Arbuscular Mycorrhizal Fungi (AM) into calcium alginate capsules of Carrizo citrange vitro-derived encapsulated microcuttings, have been carried out, in order to protect the plantlets from abiotic and biotic factors and to promote their growth during the first stages of development. Specifically, the Sinorhizobium meliloti wild type strain 1021 and its derivative RD64, that synthesizes 39-fold more IAA as compared to the wild type strain, have been used to evaluate their performances in inducing rooting of synthetic seeds.
Preliminary results on biotization of encapsulated vitro-derived propagules of Carrizo citrange [Citrus sinensis (L.) Osb. × Poncirius trifoliata (L.) Raf].
MICHELI, Maurizio;
2012
Abstract
The encapsulation technology represents a new tool to integrate micropropagation into the nursery activity. It allows to combine the advantages of zygotic or gamic seeds with those of micropropagation. One of the products of this technology is represented by synthetic seed or artificial seed, described as "artificially encapsulated somatic embryos, shoots or other tissues which can be used for sowing under in vitro or ex vitro conditions" (Aitken-Christie et al. 1995). This technique presents some problems connected to the low regrowth and conversion (formation of entire plantlet) rates of the encapsulated propagules. Synthetic seeds will be a powerful propagation tool in the nurseryman hands, if the levels of the synthetic seeds conversion will be increased also in the nurseries, without the asepsis of in vitro laboratories and with the presence of many parasitic microorganisms, like bacteria and fungi, responsible for contamination and/or for trophic competition. This research has been carried out in order to introduce the biotization to the synthetic seed technology of Carrizo citrange [C. sinensis (L.) Osb. x Poncirus trifoliata (L.) Raf.], one of the most widespread citrus rootstocks, because of its resistance to the Citrus Tristeza Virus (CTV). With this goal, preliminary experiments to set up protocols for biotization, through the introduction of Plant Growth Promoting Bacteria (PGPB) and of Arbuscular Mycorrhizal Fungi (AM) into calcium alginate capsules of Carrizo citrange vitro-derived encapsulated microcuttings, have been carried out, in order to protect the plantlets from abiotic and biotic factors and to promote their growth during the first stages of development. Specifically, the Sinorhizobium meliloti wild type strain 1021 and its derivative RD64, that synthesizes 39-fold more IAA as compared to the wild type strain, have been used to evaluate their performances in inducing rooting of synthetic seeds.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
