Protein expression changes induced in thioglycolate-elicited peritoneal murine macrophages (MF) by infection with type III Group B Streptococcus (GBS) are described. Proteins from control MF and MF incubated 2 h with live or heat-inactivated GBS were separated by 2-DE. Proteins whose expression was significantly different in infected MF, as compared with control cells, were identified by MS/MS analysis. Changes in the expression level of proteins involved in both positive and negative modulation of phagocytic functions, stress response and cell death were induced in MF by GBS infection. In particular, expression of enzymes playing a key role in production of reactive oxygen species was lowered in GBS-infected MF. Significant alterations in the expression of some metabolic enzymes were also observed, most of the glycolytic and of the pentose-cycle enzymes being down-regulated in MF infected with live GBS. Finally, evidence was obtained that GBS infection affects the expression of enzymes or enzyme subunits involved in ATP synthesis and in adenine nucleotides interconversion processes.
Protein expression changes induced in murine peritoneal macrophages by Group B Streptococcus
SUSTA, Federica;CHIASSERINI, DAVIDE;FETTUCCIARI, Katia;ORVIETANI, Pier Luigi;QUOTADAMO, FLAVIA;NOCE, ROSINA;BARTOLI, Andrea;MARCONI, Pierfrancesco;CORAZZI, Lanfranco;BINAGLIA, Luciano
2010
Abstract
Protein expression changes induced in thioglycolate-elicited peritoneal murine macrophages (MF) by infection with type III Group B Streptococcus (GBS) are described. Proteins from control MF and MF incubated 2 h with live or heat-inactivated GBS were separated by 2-DE. Proteins whose expression was significantly different in infected MF, as compared with control cells, were identified by MS/MS analysis. Changes in the expression level of proteins involved in both positive and negative modulation of phagocytic functions, stress response and cell death were induced in MF by GBS infection. In particular, expression of enzymes playing a key role in production of reactive oxygen species was lowered in GBS-infected MF. Significant alterations in the expression of some metabolic enzymes were also observed, most of the glycolytic and of the pentose-cycle enzymes being down-regulated in MF infected with live GBS. Finally, evidence was obtained that GBS infection affects the expression of enzymes or enzyme subunits involved in ATP synthesis and in adenine nucleotides interconversion processes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
