IRIS - Res&Arch Institutional Research Information System - Research & Archive
Anagrafe della Ricerca dell'Università degli Studi di Perugia.
EnglishConfocal immunofluorescence analysis indicated a relatively high localization of group V secretory phospholipase A2 (GV) in the nuclei of cultured PC12 and U251 astrocytoma cells. Here, we report the biochemical evidence for the presence of a secretory PLA2 in the nuclei of neuronal and glial cells from rat brain cortex. Enzymic activity was determined using [3H]oleate labelled Escherichia coli membranes in intact nuclei and in their soluble fractions in which the specific activity was significantly more elevated. The treatment of soluble nuclear fractions with inhibitors of cytosolic Ca2+-dependent or Ca2+-independent phospholipases A2 was ineffective whereas DTT or Indoxam, a specific inhibitor of all isoforms of sPLA2, abolished enzyme activity. The enzyme was identified as group V secretory phospholipase A2 (GV) by Western blot analysis and its nucleoplasmic localization was demonstrated by CLSM.
Scheda prodotto non validato
Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo
| Titolo: | The presence of a secretory phospholipase A2 in the nuclei of neuronal and glial cells of rat brain cortex. | |
| Autori: | ||
| Data di pubblicazione: | 2007 | |
| Rivista: | ||
| Abstract: | Confocal immunofluorescence analysis indicated a relatively high localization of group V secretor...y phospholipase A2 (GV) in the nuclei of cultured PC12 and U251 astrocytoma cells. Here, we report the biochemical evidence for the presence of a secretory PLA2 in the nuclei of neuronal and glial cells from rat brain cortex. Enzymic activity was determined using [3H]oleate labelled Escherichia coli membranes in intact nuclei and in their soluble fractions in which the specific activity was significantly more elevated. The treatment of soluble nuclear fractions with inhibitors of cytosolic Ca2+-dependent or Ca2+-independent phospholipases A2 was ineffective whereas DTT or Indoxam, a specific inhibitor of all isoforms of sPLA2, abolished enzyme activity. The enzyme was identified as group V secretory phospholipase A2 (GV) by Western blot analysis and its nucleoplasmic localization was demonstrated by CLSM. | |
| Handle: | http://hdl.handle.net/11391/32343 | |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
File in questo prodotto:
Copyright © 2021