Purpose: To study the affinity of a decapeptide, KSL (W), to tooth-like material, hydroxyapatite (HA), and assess the adsorption/desorption behavior. Methods: HA discs, untreated and pretreated with human saliva, were soaked in artificial saliva containing different KSL (W) concentrations for 20 minutes at 37ºC. The adsorption / desorption of KSL (W) with HA discs was investigated and compared with other antimicrobial compounds (Chlorhexidine, Benzalkonium Chloride, and Cetylpyridinium Hydrochloride). Results: Equilibrium adsorption was achieved after 20 minutes and the amount of KSL (W) adsorbed on the untreated and pretreated discs was ~ 17% and 39% of the original amount in the solution, respectively. At 5 minutes ~ 76% and 73% of the adsorbed KSL (W) was released from untreated and pretreated HA discs, respectively. The adsorption of KSL (W) was found to be related to the concentration of KSL (W) and the number of HA discs. With untreated HA discs, the adsorption of KSL (W) and other compounds ranged as follows; Chlorhexidine, 23% > KSL (W), 17% > benzalkonium chloride, 11% >Cetylpyridinium Hydrochloride, 4.4% With treated HA discs, Chlorhexidine adsorbed to a lesser amount, ~ 8%, than KSL (W), while adsorption of CPC was negligible. This suggests that pretreatment with human saliva lowers the adsorption to HA. Conclusion: KSL (W) adsorbed to both untreated and pretreated on HA discs, and the desorption of peptide from HA was rapid. KSL (W) could be useful as an antimicrobial, antiplaque agent in a gum formulation in situation where regular brushing of teeth is not possible.

Adsorption/desorption behavior of antimicrobial decapeptide KSL(W) on hydroxyapatite (HA)

SCHOUBBEN, Aurelie Marie Madeleine;
2006

Abstract

Purpose: To study the affinity of a decapeptide, KSL (W), to tooth-like material, hydroxyapatite (HA), and assess the adsorption/desorption behavior. Methods: HA discs, untreated and pretreated with human saliva, were soaked in artificial saliva containing different KSL (W) concentrations for 20 minutes at 37ºC. The adsorption / desorption of KSL (W) with HA discs was investigated and compared with other antimicrobial compounds (Chlorhexidine, Benzalkonium Chloride, and Cetylpyridinium Hydrochloride). Results: Equilibrium adsorption was achieved after 20 minutes and the amount of KSL (W) adsorbed on the untreated and pretreated discs was ~ 17% and 39% of the original amount in the solution, respectively. At 5 minutes ~ 76% and 73% of the adsorbed KSL (W) was released from untreated and pretreated HA discs, respectively. The adsorption of KSL (W) was found to be related to the concentration of KSL (W) and the number of HA discs. With untreated HA discs, the adsorption of KSL (W) and other compounds ranged as follows; Chlorhexidine, 23% > KSL (W), 17% > benzalkonium chloride, 11% >Cetylpyridinium Hydrochloride, 4.4% With treated HA discs, Chlorhexidine adsorbed to a lesser amount, ~ 8%, than KSL (W), while adsorption of CPC was negligible. This suggests that pretreatment with human saliva lowers the adsorption to HA. Conclusion: KSL (W) adsorbed to both untreated and pretreated on HA discs, and the desorption of peptide from HA was rapid. KSL (W) could be useful as an antimicrobial, antiplaque agent in a gum formulation in situation where regular brushing of teeth is not possible.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11391/42207
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