Ejaculated spermatozoa must undergo a number of modifications before fertilizing the oocyte: among these the capacitation and the acrosome reaction. Calcium signals play an essential role in these functional and structural modifications. Mature spermatozoa have few organelles and a very small cytoplasmic volume but maintain the homeostasis of [Ca(2+)](c) with great accuracy. We study Ca(2+) mobilization in human spermatozoa exposed to FSH and progesterone by measuring the [Ca(2+)](c) with the FURA-2AM method and report for the first time that the exposure to FSH (up to 98ng/ml) produced an increase of [Ca(2+)](c) to an extent comparable to that observed with 1muM progesterone. FSH and progesterone increase the spermatozoa [Ca(2+)](c) by acting primarily on calcium entry from the external medium. The effects of the two hormones on [Ca(2+)](c) were similar but not identical; the pre-treatment of progesterone blocks the effects of FSH, but not vice-versa. The increase of [Ca(2+)](c) due to FSH was more sensitive to nifedipine (VOCCs inhibitor) than that of progesterone. The effects of these hormones on calcium homeostasis may be relevant for sperm activation.
Effect of FSH and progesterone on human spermatozoa cytosolic calcium.
ARIENTI, Giuseppe;MAZZONI, MICHELA;SACCARDI, Carla;PALMERINI, Carlo Alberto
2010
Abstract
Ejaculated spermatozoa must undergo a number of modifications before fertilizing the oocyte: among these the capacitation and the acrosome reaction. Calcium signals play an essential role in these functional and structural modifications. Mature spermatozoa have few organelles and a very small cytoplasmic volume but maintain the homeostasis of [Ca(2+)](c) with great accuracy. We study Ca(2+) mobilization in human spermatozoa exposed to FSH and progesterone by measuring the [Ca(2+)](c) with the FURA-2AM method and report for the first time that the exposure to FSH (up to 98ng/ml) produced an increase of [Ca(2+)](c) to an extent comparable to that observed with 1muM progesterone. FSH and progesterone increase the spermatozoa [Ca(2+)](c) by acting primarily on calcium entry from the external medium. The effects of the two hormones on [Ca(2+)](c) were similar but not identical; the pre-treatment of progesterone blocks the effects of FSH, but not vice-versa. The increase of [Ca(2+)](c) due to FSH was more sensitive to nifedipine (VOCCs inhibitor) than that of progesterone. The effects of these hormones on calcium homeostasis may be relevant for sperm activation.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
