Epigenetic modifications regulate developmental genes involved in stem cell identity and lineage choice. NFI-A is a post-transcriptional microRNA-223 (miR-223) target directing human hematopoietic progenitor lineage decision: NFI-A induction or silencing boosts erythropoiesis or granulopoiesis, respectively. Here, we show that NFI-A promoter silencing, which allows granulopoiesis, is guaranteed by epigenetic events including the resolution of opposing chromatin "bivalent domains", hypermethylation, recruitment of polycomb (PcG)-RNAi complexes and by miR-223 promoter targeting activity. During granulopoiesis, miR-223 localizes inside the nucleus and targets the NFI-A promoter region containing PcGs binding sites and miR-223 complementary DNA sequences, evolutionarily conserved in mammalians. Remarkably, both the integrity of the PcGs-RNAi complex and DNA sequences matching the seed region of miR-223 are required to induce NFI-A transcriptional silencing. Moreover, ectopic miR-223 expression in human myeloid progenitors causes heterochromatic repression of NFI-A gene and channels granulopoiesis, while its stable knock-down produces opposite effects. Our findings indicate that, besides the regulation of translation of mRNA targets, endogenous miRs can affect gene expression at the transcriptional level, functioning in a critical interface between chromatin remodeling complexes and the genome to direct fate lineage determination of hematopoietic progenitors.

Polycombs and microRNA-223 regulate human granulopoiesis by transcriptional control of target gene expression.

BILLI, MONIA;RACANICCHI, SERENA;GRIGNANI, Francesco;
2012

Abstract

Epigenetic modifications regulate developmental genes involved in stem cell identity and lineage choice. NFI-A is a post-transcriptional microRNA-223 (miR-223) target directing human hematopoietic progenitor lineage decision: NFI-A induction or silencing boosts erythropoiesis or granulopoiesis, respectively. Here, we show that NFI-A promoter silencing, which allows granulopoiesis, is guaranteed by epigenetic events including the resolution of opposing chromatin "bivalent domains", hypermethylation, recruitment of polycomb (PcG)-RNAi complexes and by miR-223 promoter targeting activity. During granulopoiesis, miR-223 localizes inside the nucleus and targets the NFI-A promoter region containing PcGs binding sites and miR-223 complementary DNA sequences, evolutionarily conserved in mammalians. Remarkably, both the integrity of the PcGs-RNAi complex and DNA sequences matching the seed region of miR-223 are required to induce NFI-A transcriptional silencing. Moreover, ectopic miR-223 expression in human myeloid progenitors causes heterochromatic repression of NFI-A gene and channels granulopoiesis, while its stable knock-down produces opposite effects. Our findings indicate that, besides the regulation of translation of mRNA targets, endogenous miRs can affect gene expression at the transcriptional level, functioning in a critical interface between chromatin remodeling complexes and the genome to direct fate lineage determination of hematopoietic progenitors.
2012
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11391/825698
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