1. N-acetyl-β-d-hexosaminidase(EC 3.2.1.30) active against both p-nitrophenyl-β-2-acetamido-2-deoxy-d-glucopyranoside and p-nit the intestinal mucosa of newborn piglets. 2. The activity obtained in soluble form from homogenates prepared in various buffered media was separated by gel nitration into up to four distinct sizes with apparent molecular weights of about 15,000, 160,000, 250,000 and 500,000. 3. All sizes were active against both substrates and had similar kinetic properties. 4. The relative proportion of each size varied between individual piglets and was determined in part by the composition of the isolation media.
Unusual molecular weight forms of N-acetyl-beta-D-hexosaminidase from intestinal mucosa of newborn pigs
ORLACCHIO, Aldo;PORCELLATI, Giuseppe
1979
Abstract
1. N-acetyl-β-d-hexosaminidase(EC 3.2.1.30) active against both p-nitrophenyl-β-2-acetamido-2-deoxy-d-glucopyranoside and p-nit the intestinal mucosa of newborn piglets. 2. The activity obtained in soluble form from homogenates prepared in various buffered media was separated by gel nitration into up to four distinct sizes with apparent molecular weights of about 15,000, 160,000, 250,000 and 500,000. 3. All sizes were active against both substrates and had similar kinetic properties. 4. The relative proportion of each size varied between individual piglets and was determined in part by the composition of the isolation media.File in questo prodotto:
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