Due to the well established difference in the pharmaco/toxicological profile of many amino acid enantiomers, and also for typifying the food quality and origin, the exact knowledge of their presence and relative ratio in foodstuffs, is a matter of growing interest. In this setting, with an interest in identifying the presence of D-amino acids in a selected set of cheese samples, and with the aim to introduce a fast and easily accessible chromatographic procedure, we analyzed six cheese extracts with a CLEC-based chiral stationary phase (CLEC-CSP). The CLEC analyses were run without any pre- or post-column derivatization of the amino acidic mixture. The successful chemo-and enantioseparation were contemporarily achieved with the use of a dynamically coated CSP (C-CSP) based on the S-trityl-L-cysteine (L-STC) as the chiral selector. With the applied CLEC procedure, the presence of D-Ala, D-Asp and D-Glu was diagnosed in all the analyzed samples and then confirmed via conventional chiral gas chromatographic (CGC) analysis. A certain degree of peak overlapping was found to be the main drawback of the simplified sample analysis, which is nevertheless balanced by the advantages of the rapid detection.
Rapid Detection of D-amino Acids in Cheese with a Chiral Ligand-Exchange Chromatography System
SARDELLA, Roccaldo;IANNI, FEDERICA;NATALINI, Benedetto;
2012
Abstract
Due to the well established difference in the pharmaco/toxicological profile of many amino acid enantiomers, and also for typifying the food quality and origin, the exact knowledge of their presence and relative ratio in foodstuffs, is a matter of growing interest. In this setting, with an interest in identifying the presence of D-amino acids in a selected set of cheese samples, and with the aim to introduce a fast and easily accessible chromatographic procedure, we analyzed six cheese extracts with a CLEC-based chiral stationary phase (CLEC-CSP). The CLEC analyses were run without any pre- or post-column derivatization of the amino acidic mixture. The successful chemo-and enantioseparation were contemporarily achieved with the use of a dynamically coated CSP (C-CSP) based on the S-trityl-L-cysteine (L-STC) as the chiral selector. With the applied CLEC procedure, the presence of D-Ala, D-Asp and D-Glu was diagnosed in all the analyzed samples and then confirmed via conventional chiral gas chromatographic (CGC) analysis. A certain degree of peak overlapping was found to be the main drawback of the simplified sample analysis, which is nevertheless balanced by the advantages of the rapid detection.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
