SFX Get it!(opens in a new window)|View at Publisher| Export | Download | More... Cell Proliferation Volume 45, Issue 3, June 2012, Pages 279-285 Evaluation of phototoxic potential of aerial components of the fig tree against human melanoma (Article) Conforti, F.a , Menichini, G.b, Zanfini, L.a, Tundis, R.a, Statti, G.A.a, Provenzano, E.c, Menichini, F.a, Somma, F.b, Alfano, C.b a Department of Pharmaceutical Sciences, Faculty of Pharmacy Nutrition and Health Sciences, University of Calabria, Rende (CS), Italy b Department of Plastic and Reconstructive Surgery, Perugia University, Perugia, Italy c Operative Unit of Dermatology, A.O. of Cosenza, Cosenza, Italy View additional affiliations View references (30) Abstract Objectives: To date, Ficus carica L. cultivar Dottato (F. carica) has not been studied from a phototoxic point of view. In the present work, aerial components of F. carica from Italy, were examined to assess their antioxidant and phototoxic activity on human melanoma cells. A relationship between antioxidant, phototoxic activities and chemical composition has also been investigated. Materials and methods: Coumarin and fatty acid content in F. carica leaves, bark and woody parts were examined and compared by capillary GC and GC/MS. Polyphenolic content was also determined. Linoleic acid peroxidation and DPPH test were used to assess antioxidant activities, and MTT assay was used to evaluate anti-proliferative activity, on C32 human melanoma cells, after irradiation with a UVA dose of 1.08 J/cm 2. Results: Leaves demonstrated the best antioxidant and anti-proliferative activity in comparison to bark and wood. In particular, leaves were shown to possess the highest anti-radical activity and inhibition of peroxidation, with IC 50 values of 64 and 1.48 μg/ml respectively. The leaves had highest anti-proliferative activity with IC 50 value of 3.92 μg/ml. The phytochemical investigation revealed different composition between the coumarins, psoralen and bergapten, fatty acids, polyphenols and flavonoid content among plant parts. Conclusions: Data obtained indicate that this type of fig tree may constitute an excellent source of bioactive compounds, such as phenolics, coumarins and fatty acids. This study offers a new perspective in developing others formulations potentially useful in photodynamic therapy for treatment of non-melanoma skin cancers

Evaluation of phototoxic potential of aerial components of the fig tree against human melanoma.

ALFANO, Carmine
2012

Abstract

SFX Get it!(opens in a new window)|View at Publisher| Export | Download | More... Cell Proliferation Volume 45, Issue 3, June 2012, Pages 279-285 Evaluation of phototoxic potential of aerial components of the fig tree against human melanoma (Article) Conforti, F.a , Menichini, G.b, Zanfini, L.a, Tundis, R.a, Statti, G.A.a, Provenzano, E.c, Menichini, F.a, Somma, F.b, Alfano, C.b a Department of Pharmaceutical Sciences, Faculty of Pharmacy Nutrition and Health Sciences, University of Calabria, Rende (CS), Italy b Department of Plastic and Reconstructive Surgery, Perugia University, Perugia, Italy c Operative Unit of Dermatology, A.O. of Cosenza, Cosenza, Italy View additional affiliations View references (30) Abstract Objectives: To date, Ficus carica L. cultivar Dottato (F. carica) has not been studied from a phototoxic point of view. In the present work, aerial components of F. carica from Italy, were examined to assess their antioxidant and phototoxic activity on human melanoma cells. A relationship between antioxidant, phototoxic activities and chemical composition has also been investigated. Materials and methods: Coumarin and fatty acid content in F. carica leaves, bark and woody parts were examined and compared by capillary GC and GC/MS. Polyphenolic content was also determined. Linoleic acid peroxidation and DPPH test were used to assess antioxidant activities, and MTT assay was used to evaluate anti-proliferative activity, on C32 human melanoma cells, after irradiation with a UVA dose of 1.08 J/cm 2. Results: Leaves demonstrated the best antioxidant and anti-proliferative activity in comparison to bark and wood. In particular, leaves were shown to possess the highest anti-radical activity and inhibition of peroxidation, with IC 50 values of 64 and 1.48 μg/ml respectively. The leaves had highest anti-proliferative activity with IC 50 value of 3.92 μg/ml. The phytochemical investigation revealed different composition between the coumarins, psoralen and bergapten, fatty acids, polyphenols and flavonoid content among plant parts. Conclusions: Data obtained indicate that this type of fig tree may constitute an excellent source of bioactive compounds, such as phenolics, coumarins and fatty acids. This study offers a new perspective in developing others formulations potentially useful in photodynamic therapy for treatment of non-melanoma skin cancers
2012
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11391/912999
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