Tissue slices from the superior colliculi (SC) of the rabbit were superfused and investigated 1 week after unilateral eye removal. Amino acid levels were determined both in the tissue slices and in the medium after chemical depolarisation (56 mM K). The amino acid determinations were done fluorimetrically by precolumn derivation and HPLC separation. Colliculi contralateral to the enucleation exhibited a 16% reduction in glutamate compared with the ipsilateral colliculi. The Ca-dependent release of glutamate or other amino acids tested was not appreciably affected by enucleation. However, both the total and the Ca-independent release of glutamate was lower from contralateral SC slices compared with the ipsilateral slices. The results do not favour glutamate as the major optic nerve transmitter in the rabbit, but do not rule out glutamate as a transmitter in a minor population of retinal fibres. Key Words: Amino acid release-Retinal ablation-Superior colliculus. Sandberg M. and Corazzi L. Release of endogenous amino acids from superior colliculus of the rabbit: In vitro studies after retinal ablation. J. Neurochem. 40, 917-921 (1983).
Release of Endogenous Amino Acids from SuperiorColliculus of the Rabbit: In Vitro Studies AfterRetinal AblationMats
CORAZZI, Lanfranco
1983
Abstract
Tissue slices from the superior colliculi (SC) of the rabbit were superfused and investigated 1 week after unilateral eye removal. Amino acid levels were determined both in the tissue slices and in the medium after chemical depolarisation (56 mM K). The amino acid determinations were done fluorimetrically by precolumn derivation and HPLC separation. Colliculi contralateral to the enucleation exhibited a 16% reduction in glutamate compared with the ipsilateral colliculi. The Ca-dependent release of glutamate or other amino acids tested was not appreciably affected by enucleation. However, both the total and the Ca-independent release of glutamate was lower from contralateral SC slices compared with the ipsilateral slices. The results do not favour glutamate as the major optic nerve transmitter in the rabbit, but do not rule out glutamate as a transmitter in a minor population of retinal fibres. Key Words: Amino acid release-Retinal ablation-Superior colliculus. Sandberg M. and Corazzi L. Release of endogenous amino acids from superior colliculus of the rabbit: In vitro studies after retinal ablation. J. Neurochem. 40, 917-921 (1983).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
