A sensitive and precise high performance liquid chromatographic method for the simultaneous quantitation of S-adenosylmethionine SAMe), and S-adenosylhomocysteine (SAH) in rat liver is described. Liver is extracted by homogenization in 1.S M: PCA. SAMe and SAH are purified from contaminating substances by chromatography on Dowex 5O W x 2 and analyzed by HPLC on conventional styrene-type anion exchanger. Analytical chromatography, performed under isocratic elution conditions, is achieved in 18 min. SAH is determined as such while SAMe as adenine after hydrolysis at 60° C, under alkaline conditions. The sensitivity of the analytical procedure allows the determination of SAM and SAH in less than 200 mg of liver sample.
High performance liquid chromatographic analysis of S-adenosyl methionine and S-adenosyl homocysteine in the rat liver
FINI, Carlo;PALMERINI, Carlo Alberto;
1979
Abstract
A sensitive and precise high performance liquid chromatographic method for the simultaneous quantitation of S-adenosylmethionine SAMe), and S-adenosylhomocysteine (SAH) in rat liver is described. Liver is extracted by homogenization in 1.S M: PCA. SAMe and SAH are purified from contaminating substances by chromatography on Dowex 5O W x 2 and analyzed by HPLC on conventional styrene-type anion exchanger. Analytical chromatography, performed under isocratic elution conditions, is achieved in 18 min. SAH is determined as such while SAMe as adenine after hydrolysis at 60° C, under alkaline conditions. The sensitivity of the analytical procedure allows the determination of SAM and SAH in less than 200 mg of liver sample.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.