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EnglishA sensitive and precise high performance liquid chromatographic method for the simultaneous quantitation of S-adenosylmethionine SAMe), and S-adenosylhomocysteine (SAH) in rat liver is described. Liver is extracted by homogenization in 1.S M: PCA. SAMe and SAH are purified from contaminating substances by chromatography on Dowex 5O W x 2 and analyzed by HPLC on conventional styrene-type anion exchanger. Analytical chromatography, performed under isocratic elution conditions, is achieved in 18 min. SAH is determined as such while SAMe as adenine after hydrolysis at 60° C, under alkaline conditions. The sensitivity of the analytical procedure allows the determination of SAM and SAH in less than 200 mg of liver sample.
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| Titolo: | High performance liquid chromatographic analysis of S-adenosyl methionine and S-adenosyl homocysteine in the rat liver | |
| Autori: | ||
| Data di pubblicazione: | 1979 | |
| Rivista: | ||
| Abstract: | A sensitive and precise high performance liquid chromatographic method for the simultaneous quant...itation of S-adenosylmethionine SAMe), and S-adenosylhomocysteine (SAH) in rat liver is described. Liver is extracted by homogenization in 1.S M: PCA. SAMe and SAH are purified from contaminating substances by chromatography on Dowex 5O W x 2 and analyzed by HPLC on conventional styrene-type anion exchanger. Analytical chromatography, performed under isocratic elution conditions, is achieved in 18 min. SAH is determined as such while SAMe as adenine after hydrolysis at 60° C, under alkaline conditions. The sensitivity of the analytical procedure allows the determination of SAM and SAH in less than 200 mg of liver sample. | |
| Handle: | http://hdl.handle.net/11391/916771 | |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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