IRIS - Res&Arch Institutional Research Information System - Research & Archive
Anagrafe della Ricerca dell'Università degli Studi di Perugia.
EnglishA sensitive and precise high performance liquid chromatographic method for the simultaneous quantitation of S-adenosylmethionine SAMe), and S-adenosylhomocysteine (SAH) in rat liver is described. Liver is extracted by homogenization in 1.S M: PCA. SAMe and SAH are purified from contaminating substances by chromatography on Dowex 5O W x 2 and analyzed by HPLC on conventional styrene-type anion exchanger. Analytical chromatography, performed under isocratic elution conditions, is achieved in 18 min. SAH is determined as such while SAMe as adenine after hydrolysis at 60° C, under alkaline conditions. The sensitivity of the analytical procedure allows the determination of SAM and SAH in less than 200 mg of liver sample.
Scheda prodotto non validato
Attenzione! I dati visualizzati non sono stati sottoposti a validazione da parte dell'ateneo
| Titolo: | High performance liquid chromatographic analysis of S-adenosyl methionine and S-adenosyl homocysteine in the rat liver |
| Autori: | |
| Data di pubblicazione: | 1979 |
| Rivista: | |
| Abstract: | A sensitive and precise high performance liquid chromatographic method for the simultaneous quant...itation of S-adenosylmethionine SAMe), and S-adenosylhomocysteine (SAH) in rat liver is described. Liver is extracted by homogenization in 1.S M: PCA. SAMe and SAH are purified from contaminating substances by chromatography on Dowex 5O W x 2 and analyzed by HPLC on conventional styrene-type anion exchanger. Analytical chromatography, performed under isocratic elution conditions, is achieved in 18 min. SAH is determined as such while SAMe as adenine after hydrolysis at 60° C, under alkaline conditions. The sensitivity of the analytical procedure allows the determination of SAM and SAH in less than 200 mg of liver sample. |
| Handle: | http://hdl.handle.net/11391/916771 |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
File in questo prodotto:
Copyright © 2020