Transplantation of mesenchymal stem cells (MSCs) is a promising therapy and it is known that, in the horse, these cells are already used for teno-desmic injuries treatment. However, till now, knowledge about the MSC behaviour is scarce (Koch, Can.Vet.J., 50, 2009) and, in particular, data on the effects of the cell manipulation before transplantation are lacking. In this work we studied MSCs maintained at different temperatures for different periods of time simulating the phase of conservation before their use. Cryopreserved MSCs were thawed, resuspended in a medium devoid of fetal calf serum (1x106cc/ml) and divided in five aliquots. After a maintenance at 4°C or at room temperature for 0, 24 and 48 hours, MSCs were used for the following trials: cell viability, duplication time, Colony-Forming Unit Assays, karyotype evaluation, adipogenic and osteogenic differentiation, expression of MSC marker CD44 and CD90. Result evaluation seems to suggest that all tested manipulation were unable to induce appreciable modifications on MSC behaviour. All the aliquots were clonogenic, could differentiate along the osteogenic and adipogenic lineage and expressed high levels of CD44 and CD90 antigens. We suppose that the tested conditions of maintenance do not change the characteristics of horse adipose-derived MSCs as regards the considered parameters.
CYTOMORPHOLOGICAL EVALUATION OF HORSE ADIPOSE-DERIVED MESENCHYMAL STEM CELLS AFTER DIFFERENT STORAGE CONDITIONS.
MERCATI, FRANCESCA;CECCARELLI, Piero
2011
Abstract
Transplantation of mesenchymal stem cells (MSCs) is a promising therapy and it is known that, in the horse, these cells are already used for teno-desmic injuries treatment. However, till now, knowledge about the MSC behaviour is scarce (Koch, Can.Vet.J., 50, 2009) and, in particular, data on the effects of the cell manipulation before transplantation are lacking. In this work we studied MSCs maintained at different temperatures for different periods of time simulating the phase of conservation before their use. Cryopreserved MSCs were thawed, resuspended in a medium devoid of fetal calf serum (1x106cc/ml) and divided in five aliquots. After a maintenance at 4°C or at room temperature for 0, 24 and 48 hours, MSCs were used for the following trials: cell viability, duplication time, Colony-Forming Unit Assays, karyotype evaluation, adipogenic and osteogenic differentiation, expression of MSC marker CD44 and CD90. Result evaluation seems to suggest that all tested manipulation were unable to induce appreciable modifications on MSC behaviour. All the aliquots were clonogenic, could differentiate along the osteogenic and adipogenic lineage and expressed high levels of CD44 and CD90 antigens. We suppose that the tested conditions of maintenance do not change the characteristics of horse adipose-derived MSCs as regards the considered parameters.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
