S-2-Hydroxyacylglutathione hydrolase (Glyoxalase II) from calf brain has been purified 8333-times compared to 65,000 g supernatant of brain homogenate. The purification procedure employs Affi-Gel blue and preparative isoelectric focussing and offers a suitable method for the preparation of highly purified enzyme. Calf brain Glyoxalase II is a basic protein with a pl of 7.63 determined by isoelectric focusing. An evaluation of the relative molecular mass by gel filtration gave a value of about 23,000. During the purification procedure a constant Km value of about 0.325 mM was observed. A turnover number of 16,100 min-1 was calculated for the purified enzyme.



Titolo: Purification of S-2-hydroxyacylglutathione hydrolase (Glyoxalase II) from calf brain.
Autori: 
ROSI, Gabriella
TALESA, Vincenzo Nicola
GIOVANNINI, Elvio
mostra contributor esterni 
Data di pubblicazione: 1984
Rivista: 
BIOCHEMISTRY INTERNATIONAL  
Citazione: Purification of S-2-hydroxyacylglutathione hydrolase (Glyoxalase II) from calf brain. / Principato GB.; Rosi G.; Talesa V.; Bocchini V.; Giovannini E.. - In: BIOCHEMISTRY INTERNATIONAL. - ISSN 0158-5231. - 9/1984:3(1984), pp. 351-359.
Abstract: S-2-Hydroxyacylglutathione hydrolase (Glyoxalase II) from calf brain has been purified 8333-times compared to 65,000 g supernatant of brain homogenate. The purification procedure employs Affi-Gel blue and preparative isoelectric focussing and offers a suitable method for the preparation of highly purified enzyme. Calf brain Glyoxalase II is a basic protein with a pl of 7.63 determined by isoelectric focusing. An evaluation of the relative molecular mass by gel filtration gave a value of about 23,000. During the purification procedure a constant Km value of about 0.325 mM was observed. A turnover number of 16,100 min-1 was calculated for the purified enzyme.
Handle: http://hdl.handle.net/11391/920111
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