A method for the determination of Flavin Adenine Dinucleotide in whole blood is presented. The method comprises extraction of the vitamer by using a 6% trichloroacetic acid-20% acetonitrile mixture and direct analysis of the sample extract by using a straight-phase high performance liquid chromatography. A linear calibration curve was obtained for standard vitamer and a satisfactory recovery was observed from spiked samples. Evidence for reproducibility and precision of the assay is presented. The method was applied to determine vitamer levels in blood of healthy subjects.
High performance liquid chromatographic analysis of flavin adenine dinucleotide in whole blood
PALMERINI, Carlo Alberto;FINI, Carlo;
1985
Abstract
A method for the determination of Flavin Adenine Dinucleotide in whole blood is presented. The method comprises extraction of the vitamer by using a 6% trichloroacetic acid-20% acetonitrile mixture and direct analysis of the sample extract by using a straight-phase high performance liquid chromatography. A linear calibration curve was obtained for standard vitamer and a satisfactory recovery was observed from spiked samples. Evidence for reproducibility and precision of the assay is presented. The method was applied to determine vitamer levels in blood of healthy subjects.File in questo prodotto:
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