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Anagrafe della Ricerca dell'Università degli Studi di Perugia.
EnglishTo improve tumor targeting in a subset of patients, where tumor cells do not express the well-known tumor antigens widely used in immunotherapy, we have developed a novel biotechnological tool. It is useful for tumors of various origins for the identification of tumor-associated proteins, which are differentially expressed in tumor cells with respect to normal tissue, and exposed on the cell surface. For this purpose, a combination of techniques, such as ‘‘suppression subtractive hybridization’’ and ‘‘transmembrane trapping,’’ was employed. In applying this novel approach to breast cancer, we identified a large panel of cDNA fragments encoding for the well-known tumor-associated surface antigens, such as erb-B2, erbB3 and the urokinase receptor and, more importantly, for several clones overexpressed in breast cancer, whose cDNA fragments match the sequences of hypothetical trans- membrane proteins with unknown function. The latter may repre- sent novel tumor-specific targets.
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| Titolo: | A novel approach for identification of tumor-associated antigens expressed on the surface of tumor cells |
| Autori: | |
| Data di pubblicazione: | 2007 |
| Rivista: | |
| Abstract: | To improve tumor targeting in a subset of patients, where tumor cells do not express the well-known tumor antigens widely used in immunotherapy, we have developed a novel biotechnological tool. It is useful for tumors of various origins for the identification of tumor-associated proteins, which are differentially expressed in tumor cells with respect to normal tissue, and exposed on the cell surface. For this purpose, a combination of techniques, such as ‘‘suppression subtractive hybridization’’ and ‘‘transmembrane trapping,’’ was employed. In applying this novel approach to breast cancer, we identified a large panel of cDNA fragments encoding for the well-known tumor-associated surface antigens, such as erb-B2, erbB3 and the urokinase receptor and, more importantly, for several clones overexpressed in breast cancer, whose cDNA fragments match the sequences of hypothetical trans- membrane proteins with unknown function. The latter may repre- sent novel tumor-specific targets. |
| Handle: | http://hdl.handle.net/11391/920907 |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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