The differential staining cytotoxicity (DiSC) assay was used to evaluate the in vitro sensitivity of tumour and normal bone‐marrow cells from 21 multiple myeloma (MM) patients to antitumour agents methylprednisolone (PDN), nitrogen mustard (NM) and recombinant interferon α‐2b (IFNα) tested singly and in the combinations PDN+IFNα and NM+IFNα. Both the PDN‐IFNα and NM‐IFNα associations were more efficacious than any agents used singly in reducing the percentage of myeloma cell survival. However, whereas NM, alone and in combination with IFNα, provoked a severe reduction in normal bone‐marrow population, PDN and PDN+IFNα induced an increase percentage survival of normal bone‐marrow cells. These findings indicate that, at least in vitro, the PDN‐IFNα combination exerts a great antitumour effect which is not associated with a relevant cytotoxic activity on normal myeloid cells. Copyright © 1991 John Wiley & Sons, Ltd
In vitro synergistic activity of PDN-IFN alpha and NM + IFN alpha combinations on fresh bone-marrow samples from multiple myeloma patients.
LIBERATI, Anna Marina
1991
Abstract
The differential staining cytotoxicity (DiSC) assay was used to evaluate the in vitro sensitivity of tumour and normal bone‐marrow cells from 21 multiple myeloma (MM) patients to antitumour agents methylprednisolone (PDN), nitrogen mustard (NM) and recombinant interferon α‐2b (IFNα) tested singly and in the combinations PDN+IFNα and NM+IFNα. Both the PDN‐IFNα and NM‐IFNα associations were more efficacious than any agents used singly in reducing the percentage of myeloma cell survival. However, whereas NM, alone and in combination with IFNα, provoked a severe reduction in normal bone‐marrow population, PDN and PDN+IFNα induced an increase percentage survival of normal bone‐marrow cells. These findings indicate that, at least in vitro, the PDN‐IFNα combination exerts a great antitumour effect which is not associated with a relevant cytotoxic activity on normal myeloid cells. Copyright © 1991 John Wiley & Sons, LtdI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
