Findings obtained using different approaches indicated the occurrence of genomic size variations within V. faba. Significant differences in the basic amount of nuclear DNA (up to 34.6 per cent) between 39 local populations collected from the Mediterranean Basin were observed by means of Feulgen/DNA cytophotometry. By contrast, no difference in genome size was found when five commercial varieties were compared. Dot blot hybridization of FokI V. faba repeats to genomic DNAs showed up to fourfold differences in the redundancy of these sequences in the nuclear DNA of different accessions. In agreement with the cytophotometric findings, a significant, positive correlation was determined between the DNA contents of populations and the copy numbers of DNA sequences related to FokI repeats. Significant differences between accessions were found in the length of the chromosome complement at metaphase, and these differences were particularly apparent in certain chromosome pairs. A positive correlation was found between the length of the complement and the genome size of the populations. These results are discussed in relation to other data in the literature on intraspecific nuclear DNA changes.
Genome size variation in Vicia faba.
CECCARELLI, Marilena;CIONINI, Pier Giorgio
1995
Abstract
Findings obtained using different approaches indicated the occurrence of genomic size variations within V. faba. Significant differences in the basic amount of nuclear DNA (up to 34.6 per cent) between 39 local populations collected from the Mediterranean Basin were observed by means of Feulgen/DNA cytophotometry. By contrast, no difference in genome size was found when five commercial varieties were compared. Dot blot hybridization of FokI V. faba repeats to genomic DNAs showed up to fourfold differences in the redundancy of these sequences in the nuclear DNA of different accessions. In agreement with the cytophotometric findings, a significant, positive correlation was determined between the DNA contents of populations and the copy numbers of DNA sequences related to FokI repeats. Significant differences between accessions were found in the length of the chromosome complement at metaphase, and these differences were particularly apparent in certain chromosome pairs. A positive correlation was found between the length of the complement and the genome size of the populations. These results are discussed in relation to other data in the literature on intraspecific nuclear DNA changes.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
