The Inhibitory Effect of Chitosan Based Films, Incorporated with Essential Oil of Perilla frutescens Leaves, against Botrytis cinerea during the Storage of Strawberries

Reduction in food waste, as well as non-invasive methods for extending the shelf-life of perishable fruits, are important global challenges. To achieve these objectives, in this paper, the use of natural compounds, chitosan films (CS) incorporated with essential oils from leaves, for postharvest fungal protection of strawberries is proposed. In the present study, the CS films incorporated with the essential oil from Perilla frutescens leaves (PFEO) at different concentrations were prepared and employed for packaging strawberries infected by B. cinerea during refrigerated storage at 4◦ C for 10 days. Interestingly, the strawberries coated with CS films containing PFEO at 1.0% during this period possessed an effective antimicrobial effect against B. cinerea infection in potato dextrose agar (PDA). Moreover, the quality properties of the strawberries, (i.e., weight loss, firmness index, decay percentage, yeasts/molds, pH value, total soluble solids, titrable acidity, and maturity index), together with the sensory attributes (i.e., appearance, flavor, taste, and overall acceptability (p < 0.05 or p < 0.01)) were improved. These results demonstrated that (i) PFEO displayed a significant inhibitory effect against B. cinerea infection in strawberries, (ii) CS films containing PFEO at 1.0% could be a sustainable active food packaging for the refrigerated storage of strawberries.