Conformational changes in the unfolding process of lysozyme in water and ethanol/water solutions

Sassi, Paola; Onori, G.; Giugliarelli, Alessandra; Paolantoni, Marco; Cinelli, S.; Morresi, Assunta

doi:10.1016/j.molliq.2010.12.008

In the present study, the thermal unfolding of lysozyme in D(2)O and D(2)O/CH(3)CH(2)OD solutions is analyzed by means of FTIR spectroscopy. A detailed investigation of the conformational rearrangement of the protein upon heating is achieved by inspection of the amide bands. The effects of partial and total deuteration on amide groups are also investigated for they suggest important information about exposure to the solvent. Finally, a comparison between the characteristics of the unfolding process of lysozyme in water and in ethanol/water solutions is performed to clarify some misinterpretation about the possible formation of intermediates.

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Titolo:	Conformational changes in the unfolding process of lysozyme in water and ethanol/water solutions
Autori:	SASSI, Paola G. Onori GIUGLIARELLI, ALESSANDRA PAOLANTONI, Marco S. Cinelli MORRESI, Assunta mostra contributor esterni nascondi contributor esterni
Data di pubblicazione:	2011
Rivista:	JOURNAL OF MOLECULAR LIQUIDS
Abstract:	In the present study, the thermal unfolding of lysozyme in D(2)O and D(2)O/CH(3)CH(2)OD solutions... is analyzed by means of FTIR spectroscopy. A detailed investigation of the conformational rearrangement of the protein upon heating is achieved by inspection of the amide bands. The effects of partial and total deuteration on amide groups are also investigated for they suggest important information about exposure to the solvent. Finally, a comparison between the characteristics of the unfolding process of lysozyme in water and in ethanol/water solutions is performed to clarify some misinterpretation about the possible formation of intermediates.
Handle:	http://hdl.handle.net/11391/176628
Appare nelle tipologie:	1.1 Articolo in rivista

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