Araucaria araucana (Mol.) K. Koch is one of the most important native species of Chile and Argentina, and also one of the most endangered. In this study, we report the development and characterization of a set of microsatellite markers in the species by means of the microsatellite-AFLP (M-AFLP) technique. A total of 25 M-AFLP derived bands, showing a typical microsatellite pattern, were selected and sequenced. Of these, 12 that contained microsatellite sequences, were used for primer extension. Six of the resulting SSR markers pro- vided easily interpretable patterns and were used to investigate the level of genetic diversity in two popula- tions of A. araucana. A total of 43 alleles were amplified. The mean overall loci of observed and expected het- erozygosities for the Conguillio and Villa Araucaria pop- ulations were 0.322 and 0.443, respectively. The primers presented in this study may provide useful information for the establishment of a conservation strategy in the species.
Microsatellite-AFLP development for Araucaria araucana (Mol.) K. Koch, an endangered conifer of Chilean and Argentinean native forests
MARCONI, GIANPIERO;RAGGI, LORENZO;ALBERTINI, Emidio;
2011
Abstract
Araucaria araucana (Mol.) K. Koch is one of the most important native species of Chile and Argentina, and also one of the most endangered. In this study, we report the development and characterization of a set of microsatellite markers in the species by means of the microsatellite-AFLP (M-AFLP) technique. A total of 25 M-AFLP derived bands, showing a typical microsatellite pattern, were selected and sequenced. Of these, 12 that contained microsatellite sequences, were used for primer extension. Six of the resulting SSR markers pro- vided easily interpretable patterns and were used to investigate the level of genetic diversity in two popula- tions of A. araucana. A total of 43 alleles were amplified. The mean overall loci of observed and expected het- erozygosities for the Conguillio and Villa Araucaria pop- ulations were 0.322 and 0.443, respectively. The primers presented in this study may provide useful information for the establishment of a conservation strategy in the species.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
