A method was set up to study the antigenotoxic property of the probiotic Lactobacillus rhamnosus IMC 501 (R) against 4-nitroquinoline-1-oxide (4-NQO). The method is based on liquid-liquid extraction followed by gas chromatography-mass spectrometry (GC-MS) analysis. The GC-MS analysis was used to determine the quantitative change of 4-NQO before and after co-incubation with Lactobacillus rhamnosus IMC 501 (R). The results from GC-MS analysis showed that 4-NQO genotoxicity was inhibited and then converted to less toxic compounds. These preliminary results from GC-MS analysis indicate that the method described is able to detect physicochemical modifications of the genotoxic agent, which occur after co-incubation with bacteria cells. The method, therefore, is a novel alternative to biological assays for detecting the antigenotoxicity of lactobacilli.
Investigation on antigenotoxic properties of the probiotic Lactobacillus rhamnosus IMC 501 by gaschromatography-mass spectrometry
CENCI, Giovanni;TROTTA, Francesca;CALDINI, Giovanna;
2010
Abstract
A method was set up to study the antigenotoxic property of the probiotic Lactobacillus rhamnosus IMC 501 (R) against 4-nitroquinoline-1-oxide (4-NQO). The method is based on liquid-liquid extraction followed by gas chromatography-mass spectrometry (GC-MS) analysis. The GC-MS analysis was used to determine the quantitative change of 4-NQO before and after co-incubation with Lactobacillus rhamnosus IMC 501 (R). The results from GC-MS analysis showed that 4-NQO genotoxicity was inhibited and then converted to less toxic compounds. These preliminary results from GC-MS analysis indicate that the method described is able to detect physicochemical modifications of the genotoxic agent, which occur after co-incubation with bacteria cells. The method, therefore, is a novel alternative to biological assays for detecting the antigenotoxicity of lactobacilli.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
