The genus Pleurotus (Fr.) P. Kumm (Pleurotaceae, Basidiomycota) comprises a cosmopol- itan group of mushrooms highly appreciated for their nutritional value and health-promoting ben- efits. Despite there being many studies about the phytochemical composition of Pleurotus spp., there are very few reports dealing with the phytochemistry, antioxidant and antimicrobial activities of P. columbinus Quél. In this study, a mass spectrometry ultra-performance liquid chromatography mass spectrometry (UHPLC)-QTOF method, coupled with principal component analysis (PCA), was ap- plied to the P. columbinus metabolome in order to investigate the influence of different agri-food residues as growth substrates for P. columbinus cultivation, on the bioactive chemical profile of fruit- ing bodies and evaluated their potential as antioxidants and antimicrobials. Additionally, a quanti- tative HPLC-DAD-MS analysis was conducted on phenolic and flavonoid compounds, that could explain, albeit partially, the observed biological effects of P. columbinus extracts. The qualitative metabolic profile identified 97 metabolites, whereas the quantitative HPLC-DAD-MS analysis con- firmed the presence of phenolic and flavonoids, in the mushroom extracts, which also showed in- trinsic scavenging/reducing and antimicrobial effects. The antibacterial effects were particularly ev- ident against Escherichia coli, whereas Tricophyton and Aspergillus were the dermatophytes more sen- sitive to the mushroom extracts. The present study supports more in-depth investigations, aimed at evaluating the influence of growth substrate on P. columbinus antimicrobial and antioxidant prop- erties. The extracts from P. columbinus revealed valuable sources of primary and secondary metab- olites, thus suggesting potential applications in the formulation of food supplements with biological properties, above all in terms of antioxidant and antimicrobial properties.
Metabolomic Profiling and Biological Activities of Pleurotus columbinus Quél. Cultivated on Different Agri-Food Byproducts
Angelini P.Writing – Original Draft Preparation
;Pellegrino R. M.Methodology
;Alabed H. B. R.;Ianni F.Membro del Collaboration Group
;Blasi F.Conceptualization
;Cossignani L.Conceptualization
;Venanzoni R.Supervision
;Menghini L.
;
2021
Abstract
The genus Pleurotus (Fr.) P. Kumm (Pleurotaceae, Basidiomycota) comprises a cosmopol- itan group of mushrooms highly appreciated for their nutritional value and health-promoting ben- efits. Despite there being many studies about the phytochemical composition of Pleurotus spp., there are very few reports dealing with the phytochemistry, antioxidant and antimicrobial activities of P. columbinus Quél. In this study, a mass spectrometry ultra-performance liquid chromatography mass spectrometry (UHPLC)-QTOF method, coupled with principal component analysis (PCA), was ap- plied to the P. columbinus metabolome in order to investigate the influence of different agri-food residues as growth substrates for P. columbinus cultivation, on the bioactive chemical profile of fruit- ing bodies and evaluated their potential as antioxidants and antimicrobials. Additionally, a quanti- tative HPLC-DAD-MS analysis was conducted on phenolic and flavonoid compounds, that could explain, albeit partially, the observed biological effects of P. columbinus extracts. The qualitative metabolic profile identified 97 metabolites, whereas the quantitative HPLC-DAD-MS analysis con- firmed the presence of phenolic and flavonoids, in the mushroom extracts, which also showed in- trinsic scavenging/reducing and antimicrobial effects. The antibacterial effects were particularly ev- ident against Escherichia coli, whereas Tricophyton and Aspergillus were the dermatophytes more sen- sitive to the mushroom extracts. The present study supports more in-depth investigations, aimed at evaluating the influence of growth substrate on P. columbinus antimicrobial and antioxidant prop- erties. The extracts from P. columbinus revealed valuable sources of primary and secondary metab- olites, thus suggesting potential applications in the formulation of food supplements with biological properties, above all in terms of antioxidant and antimicrobial properties.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
