Valorization of agricultural by-products and waste for the development of functional ingredients, foods, and nutraceuticals is a current field of research of great importance in the international economy [1]. The analytical characterization of the bioactive compounds in these products is a fundamental requirement to assess their quality and to identify the molecules responsible for biological activity. For the extraction of the different bioactive molecules, in addition to the conventional methods, emerging methodologies have been proposed. They provide high extraction yields and numerous technical or environmental advantages during extraction processes, such as short extraction times and use of green solvents. The extraction of chemical compounds from plant materials can be carried out using supercritical fluids or by ultrasound and microwave-assisted extraction, accelerated solvent and enzyme-assisted extraction [2]. In this presentation, the results of research activity aimed at developing methods for extraction and characterization of bioactive phenols in leaves from Olea europaea, Moringa oleifera, and Lycium species are shown. The optimization of the factors affecting phenol extraction yield in olive leaves was carried out by Experimental Design. Innovative extraction techniques were used to isolate phenolic compound from Moringa and Lycium leaves. The analytical characterization of the phenolic extracts was performed by High-Performance Liquid Chromatography hyphenated with Mass Spectrometer Detector. The biological activity was studied by in vitro assays, for example, the antioxidant capacity was evaluated by measuring the antiradical activity and the reducing capacity. Moreover, the phenolic extracts from Lycium leaves were tested for their potential inhibitory effects on pancreatic lipase and -glucosidase. Preliminary results are promising as phenolic extracts could be natural sources of enzyme inhibitors useful in obesity and diabetes control. Further research is underway to investigate the compounds and mechanisms involved in enzymatic inhibition. In conclusion, the results of this research indicate that the investigated plant materials are valuable sources of bioactive compounds with potential application in pharmaceutical, nutraceutical and cosmeceutical fields. References [1] F. Girotto, L. Alibardi, R. Cossu: Food waste generation and industrial uses: A review. Waste Management 45 (2015) 32-41. [2] J.G. Gil-Chávez, J.A. Villa, F.J. Ayala-Zavala, H.J. Basilio, D. Sepulveda, E.M. Yahia, G.A. González-Aguilar: Technologies for extraction and production of bioactive compounds to be used as nutraceuticals and food ingredients: an overview. Comprehensive Reviews in Food Science and Food Safety 12 (2013) 5-23.
Unconventional extraction and characterization of phenolic compounds for the valorization of agricultural by-products
Cossignani L.;Pollini L.;Tringaniello C.;Verducci G.;Blasi F.
2019
Abstract
Valorization of agricultural by-products and waste for the development of functional ingredients, foods, and nutraceuticals is a current field of research of great importance in the international economy [1]. The analytical characterization of the bioactive compounds in these products is a fundamental requirement to assess their quality and to identify the molecules responsible for biological activity. For the extraction of the different bioactive molecules, in addition to the conventional methods, emerging methodologies have been proposed. They provide high extraction yields and numerous technical or environmental advantages during extraction processes, such as short extraction times and use of green solvents. The extraction of chemical compounds from plant materials can be carried out using supercritical fluids or by ultrasound and microwave-assisted extraction, accelerated solvent and enzyme-assisted extraction [2]. In this presentation, the results of research activity aimed at developing methods for extraction and characterization of bioactive phenols in leaves from Olea europaea, Moringa oleifera, and Lycium species are shown. The optimization of the factors affecting phenol extraction yield in olive leaves was carried out by Experimental Design. Innovative extraction techniques were used to isolate phenolic compound from Moringa and Lycium leaves. The analytical characterization of the phenolic extracts was performed by High-Performance Liquid Chromatography hyphenated with Mass Spectrometer Detector. The biological activity was studied by in vitro assays, for example, the antioxidant capacity was evaluated by measuring the antiradical activity and the reducing capacity. Moreover, the phenolic extracts from Lycium leaves were tested for their potential inhibitory effects on pancreatic lipase and -glucosidase. Preliminary results are promising as phenolic extracts could be natural sources of enzyme inhibitors useful in obesity and diabetes control. Further research is underway to investigate the compounds and mechanisms involved in enzymatic inhibition. In conclusion, the results of this research indicate that the investigated plant materials are valuable sources of bioactive compounds with potential application in pharmaceutical, nutraceutical and cosmeceutical fields. References [1] F. Girotto, L. Alibardi, R. Cossu: Food waste generation and industrial uses: A review. Waste Management 45 (2015) 32-41. [2] J.G. Gil-Chávez, J.A. Villa, F.J. Ayala-Zavala, H.J. Basilio, D. Sepulveda, E.M. Yahia, G.A. González-Aguilar: Technologies for extraction and production of bioactive compounds to be used as nutraceuticals and food ingredients: an overview. Comprehensive Reviews in Food Science and Food Safety 12 (2013) 5-23.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
